The monarch holds her youngest great-grandchild Princess Charlotte in her arms in the photographs captured by celebrity photographer Annie Leibovitz.
Prince George, who is now two, as well as Zara Phillips's daughter Mia Tindall, who is proudly holding the Queen's black handbag, and Peter Phillips's daughters Savannah, five, and three-year-old Isla, also feature in the touching image.
She is also joined by the Earl and Countess of Wessex's children - James, Viscount Severn, eight, who has his hands in his pockets, and Lady Louise Windsor.
Lady Louise, who was one of the Duke and Duchess of Cambridge's bridesmaids, is now 12 and towers above her younger relatives. The photograph was taken in the Green Drawing Room, part of the Windsor Castle's semi-State apartments just after Easter.
Little princess? More like little madam! Duchess Catherine has revealed that her daughter Princess Charlotte’s sweet, adorable looks can be deceiving… she’s actually a feisty one!
The Duchess of Cambridge uncovered the royal secret to a group of guests at the Guildhall reception after the National Service of Thanksgiving for Queen Elizabeth’s 90th birthday at St Paul’s Cathedral.
Guests couldn’t help but remark at what a gorgeously angelic little baby Princess Charlotte was, only to have Kate set the record straight.
“As a mum myself,” said Glynis Double, wife of the city’s Remembrancer, aka head of protocol, “I asked her about her children and said what a pretty little thing we all thought Princess Charlotte was."
Squealing with excited pleasure at the balloons, bubbles and puppets, Prince George and Princess Charlotte put on a beguiling royal appearance. And when Charlotte used the occasion to utter her first recorded word in public, ‘pop’, the siblings’ official overseas tour debut was complete.
William displayed the parenting skills so rarely seen outside the privacy of his home this week in Canada. Watching the Prince at play at a children’s garden party in the grounds of Government House in Victoria, British Columbia, you recognise instinctively that this is the father - and husband - that Kate has always seen.
Prince William and Kate expressed their gratitude for the warm welcome they received from Canadians as they wrapped up their tour of Canada, a former British colony. William said the trip was memorable because it was their first official trip abroad as a family of four.
"We feel very lucky to have been able to introduce George and Charlotte to Canada," William said in a statement. "This country will play a big part in the lives of our children and we have created such happy memories for our family during this visit."